Welcome to the P[acman] Recombineering Resource Hub

About Us

Your centralized source for protocols, plasmids, genomic tools, troubleshooting guides, and educational resources related to the P[acman] recombineering system for Drosophila melanogaster.

Whether you are a researcher, genetic engineer, or student, this website provides everything you need to design, construct, and manipulate large genomic DNA fragments using BAC recombineering.

What Is P[acman] Recombineering?

P[acman] (P/ΦC31 artificial chromosome for manipulation) is an advanced technique used to:

01

Clone large genomic fragments (up to 133 kb)

02

Perform BAC recombineering using Red/ET or λ recombination

03

Generate precise transgenes using ΦC31-mediated site-specific integration

04

Study gene function, regulation, and developmental pathways in Drosophila

Recombineering-ready backbones

attB-containing P[acman] vectors

GFP/RFP tagging vectors

Tools & Resources

P[acman] Vectors Library

Access complete information for the most used vectors:

Gateway-modified versions

pBeloBAC11-based vectors

Protocols & Tutorials

BAC DNA extraction protocols

Recombineering using λ Red system

Homologous recombination primer design

Counter-selection strategies

ΦC31 site-specific integration workflow

Quality control of engineered BACs

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Key Techniques & Laboratory Tools

Diagnostic & Validation Tools

Fluorescence Imaging of Tagged Constructs

Sanger Sequencing for Junction Verification

Genomic PCR Confirmation after Integration

Restriction Digest Mapping

PCR Screening and Colony PCR

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